2JHN
3-methyladenine dna-glycosylase from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-13 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.494, 49.968, 105.798 |
| Unit cell angles | 90.00, 107.43, 90.00 |
Refinement procedure
| Resolution | 12.000 - 1.800 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.22300 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.360 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 0.500 |
| Number of reflections | 63551 | |
| <I/σ(I)> | 18 | 3.5 |
| Completeness [%] | 98.7 | 97.9 |
| Redundancy | 8 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 50 MM MES, PH6.0., pH 6.00 |
