2JHC
The structure of bluetongue virus VP4 reveals a multifunctional RNA- capping production-line
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 75.239, 75.239, 421.838 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 3.000 |
| R-factor | 0.258 |
| Rwork | 0.254 |
| R-free | 0.33300 |
| Structure solution method | OTHER |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.884 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.160 | 0.540 |
| Number of reflections | 14461 | |
| <I/σ(I)> | 16 | 1.1 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 14.6 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.7 | 100 MM IMIDAZOLE PH 8.0, 2%-8% PROPAN-2-OL, 0-250 MM NACL, 50 MM DTT |
