2JGU

crystal structure of DNA-directed DNA polymerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	92.178, 127.923, 89.210
Unit cell angles	90.00, 109.11, 90.00

Refinement procedure

Resolution	15.000 - 2.600
R-factor	0.2749
Rwork	0.275
R-free	0.27110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qqc
RMSD bond length	0.015
RMSD bond angle	2.334
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.060	0.260
Number of reflections	848408
<I/σ(I)>	18.4	1.72
Completeness [%]	93.8	85.1
Redundancy	4.9	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		pH 6.5