2JFE
The crystal structure of human cytosolic beta-glucosidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-17 |
Detector | ADSC CCD |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 90.479, 90.479, 127.925 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.640 - 2.700 |
R-factor | 0.201 |
Rwork | 0.197 |
R-free | 0.28000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1v02 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.632 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.790 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.180 | 0.670 |
Number of reflections | 17144 | |
<I/σ(I)> | 10.6 | 3.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.6 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | CRYSTALS GREW WITHIN SEVERAL DAYS IN THE CRYSTALLIZATION TRIAL CONTAINING 0.2 M AMMONIUM SULPHATE, MES BUFFER AT PH 6.5 AND 30 % (W/V) POLYETHYLENEGLYCOL-MONOMETHYLETHER (PEG-MME) 5500 |