2JES
Portal protein (gp6) from bacteriophage SPP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-01 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 174.314, 221.405, 421.866 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.810 - 3.400 |
| R-factor | 0.289 |
| Rwork | 0.288 |
| R-free | 0.31900 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.137 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.460 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.100 | 0.530 |
| Number of reflections | 111682 | |
| <I/σ(I)> | 6.9 | 3.2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 8 MG/ML PORTAL PROTEIN, 20% PEG 400, 100 MM CACL2, 50 MM HEPES PH 7.6, 10% GLYCEROL, 0.5 MM HGCL |
