2JE1
The crystal Structure of the tumor supressor protein pp32 (Anp32a) :structural insights into the Anp32 family of proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 63 |
| Unit cell lengths | 106.506, 106.506, 133.153 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.690 |
| R-factor | 0.241 |
| Rwork | 0.238 |
| R-free | 0.30300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HOMOLOGY MODEL |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.485 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.130 | 0.540 |
| Number of reflections | 23193 | |
| <I/σ(I)> | 11.1 | 3.1 |
| Completeness [%] | 97.8 | 99.3 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | pH 5.50 |
