2JDQ

C-terminal domain of influenza A virus polymerase PB2 subunit in complex with human importin alpha5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-15
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	94.970, 100.550, 151.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.200
R-factor	0.208
Rwork	0.206
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q1s
RMSD bond length	0.012
RMSD bond angle	1.273
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.090	0.690
Number of reflections	74104
<I/σ(I)>	9.4	2.4
Completeness [%]	99.5	99.9
Redundancy	4	4.06

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6		1 MICROLITRE OF PROTEIN SOLUTION, AT 20 MG/ML IN 30 MM TRIS-HCL, PH 7.5, 150 MM NACL, 3 MM BETA-MERCAPTOETHANOL WAS MIXED WITH AN EQUAL VOLUME OF RESERVOIR SOLUTION (0.1 M NAAC, PH 4.6, 5 MM CACL2, 15% MPD)