2JDJ

crystal structure of HapK from Hahella chejuensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-05
Detector	ADSC CCD
Spacegroup name	P 32 1 2
Unit cell lengths	58.306, 58.306, 137.882
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.520 - 2.000
R-factor	0.191
Rwork	0.189
R-free	0.22300
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.011
RMSD bond angle	1.305
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.080	0.290
Number of reflections	35383
<I/σ(I)>	44	8.6
Completeness [%]	100.0	100
Redundancy	11	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		1.8 M AMMONIUM SULFATE, 7% ISOPROPANOL, pH 6.5