2JCY
X-ray structure of mutant 1-deoxy-D-xylulose 5-phosphate reductoisomerase, DXR, Rv2870c, from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 110 |
| Collection date | 2006-04-06 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.109, 64.817, 86.330 |
| Unit cell angles | 90.00, 101.72, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.350 |
| R-factor | 0.183 |
| Rwork | 0.180 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c82 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.095 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.070 | 0.190 |
| Number of reflections | 30603 | |
| <I/σ(I)> | 17.5 | |
| Completeness [%] | 99.0 | 93.1 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
