2JCR
The hyaluronan binding domain of murine CD44 in a Type B complex with an HA 8-mer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-04 |
| Detector | BRUKER |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.800, 82.100, 32.400 |
| Unit cell angles | 90.00, 118.00, 90.00 |
Refinement procedure
| Resolution | 28.610 - 2.000 |
| R-factor | 0.179 |
| Rwork | 0.175 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jcq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.280 |
| Data reduction software | PROTEUM |
| Data scaling software | SADABS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.600 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.110 | 0.240 |
| Number of reflections | 9641 | |
| <I/σ(I)> | 10.4 | 3.6 |
| Completeness [%] | 92.3 | 67.5 |
| Redundancy | 2.5 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | CO-CRYSTALS OF THE CD44/HA8 COMPLEX WERE PREPARED AFTER ADDITION OF HA8 (2MM FINAL CONCENTRATION) TO THE 0.5 MM PROTEIN SOLUTION FOLLOWED BY MIXING 1:1 WITH WELL SOLUTIONS CONTAINING 25% (W/V) PEG 3350 AND 100MM NACL IN 100MM HEPES BUFFERED AT PH 8.0 |
