2JCO
Crystal structure of wild type alpha-1,3 Galactosyltransferase in the absence of ligands
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 93.570, 93.570, 108.417 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.110 - 2.570 |
| R-factor | 0.268 |
| Rwork | 0.263 |
| R-free | 0.32800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k4v |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.730 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.660 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.070 | 0.680 |
| Number of reflections | 15988 | |
| <I/σ(I)> | 24 | 2.11 |
| Completeness [%] | 98.0 | 84.8 |
| Redundancy | 18.7 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1M TRIS-HCL BUFFER PH 8.0 AND 1.4M AMMONIUM SULPHATE |
