2JCM
Crystal structure of Human Cytosolic 5'-Nucleotidase II in complex with beryllium trifluoride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-08 |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 91.480, 128.260, 131.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.670 - 2.150 |
| R-factor | 0.213 |
| Rwork | 0.210 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j2c |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.759 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0021) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.200 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.160 | 0.500 |
| Number of reflections | 41406 | |
| <I/σ(I)> | 7.14 | 3.36 |
| Completeness [%] | 98.0 | 99.8 |
| Redundancy | 5.2 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 1.8 M MAGNESIUM SULFATE, 0.1 M TRIS PH 8.5 |
