2JCB
The crystal structure of 5-formyl-tetrahydrofolate cycloligase from Bacillus anthracis (BA4489)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-05 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.152, 45.499, 66.999 |
| Unit cell angles | 74.92, 78.01, 70.14 |
Refinement procedure
| Resolution | 18.690 - 1.600 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ydm |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.628 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.040 | 0.340 |
| Number of reflections | 49797 | |
| <I/σ(I)> | 13.1 | 2.9 |
| Completeness [%] | 95.5 | 94.3 |
| Redundancy | 2.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 3.8MM FOLINATE, 3.8MM ATP, 30MM MAGNESIUM CHLORIDE, 25% POLYETHYLENE GLYCOL 3350, 100 MM BIS-TRIS (PH 5.5) |
