2JC9
Crystal structure of Human Cytosolic 5'-Nucleotidase II in complex with adenosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-08 |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 91.273, 127.983, 130.601 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.330 - 1.500 |
| R-factor | 0.162 |
| Rwork | 0.160 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j2c |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.405 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.950 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 | 0.440 |
| Number of reflections | 122218 | |
| <I/σ(I)> | 17.6 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 1.8 M MAGNESIUM SULFATE, 0.1 M TRIS PH 8.5 |
