2JC4

3'-5' exonuclease (NExo) from Neisseria Meningitidis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	46.193, 58.618, 88.635
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 1.900
Rwork	0.152
R-free	0.20200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bix
RMSD bond length	0.016
RMSD bond angle	1.501
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.090	0.320
Number of reflections	19164
<I/σ(I)>	24.5	5.1
Completeness [%]	97.6	91.4
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		PROTEIN CONCENTRATION 8.7MG/ML BUFFER: 10 MM TRIS, PH 7.0, 100 MM NACL . WELL SOLUTION: 100 MM NA CACODYLATE PH 6 200 MM NA ACETATE, 15 % PEG 8000. THE CRYSTALLISATION PLATES USED WERE CORNING ROUND BOTTOM 96 WELL PLATES. THE CRYSTALLISATION DROPS CONSISTED OF 1UL PROTEIN AND 1UL WELL SOLUTION.