2JC2
The crystal structure of the natural F112L human sorcin mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 64.385, 64.385, 314.837 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.500 - 2.500 |
| R-factor | 0.254 |
| Rwork | 0.252 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1juo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.851 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.080 | 0.600 |
| Number of reflections | 25105 | |
| <I/σ(I)> | 7 | 2 |
| Completeness [%] | 96.8 | 85.1 |
| Redundancy | 5.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.7 | AMMONIUM SULFATE 1.0M, PH 5.7, 12% V/V DIOXANE |
