2JBA
PhoB response regulator receiver domain constitutively-active double mutant D53A and Y102C.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.907, 47.631, 59.703 |
Unit cell angles | 90.00, 99.34, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.450 |
R-factor | 0.189 |
Rwork | 0.189 |
R-free | 0.20400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b00 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.309 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.700 | 1.530 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.070 | 0.290 |
Number of reflections | 41827 | |
<I/σ(I)> | 15.2 | 2.3 |
Completeness [%] | 94.5 | 70.3 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 3 MICROLITER OF PROTEIN SOLUTION AT 5.5 MG/ML AND 3 MICROLITER OF RESERVOIR SOLUTION (20% (W/V) PEG 4K, 0.4M SODIUM ACETATE, 0.1M TRISHCL (PH 8), 0.01M DTT |