2JAT
Structure of deoxyadenosine kinase from M.mycoides with products dcmp and a flexible dcdp bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 |
| Unit cell lengths | 83.931, 83.931, 53.310 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.600 |
| R-factor | 0.224 |
| Rwork | 0.222 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jas |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.269 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.040 | 0.300 |
| Number of reflections | 11196 | |
| <I/σ(I)> | 22.7 | 4.1 |
| Completeness [%] | 98.0 | 99.6 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.9 | 20% PEG 3350, 0.2 M MGCL2, pH 5.90 |
