2JAR
Crystal structure of D12N variant of mouse cytosolic 5'(3')- deoxyribonucleotidase (cdN) in complex with deoxyuridine 5'- monophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 62 |
| Unit cell lengths | 75.309, 75.309, 85.174 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 1.940 |
| R-factor | 0.158 |
| Rwork | 0.156 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.423 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.230 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.100 | 0.340 |
| Number of reflections | 20062 | |
| <I/σ(I)> | 19 | 5.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.7 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 279 | 0.2 M MAGNESIUM CHLORIDE, 0.1 M BIS-TRIS PH 5.5, 23-25% PEG3350 AT 6 DEGREES CELSIUS |
