2JAB
A designed ankyrin repeat protein evolved to picomolar affinity to Her2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-12-10 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.080, 52.191, 70.164 |
| Unit cell angles | 90.00, 105.19, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.700 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1MJ0 AND 2BKG |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.285 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.300 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.120 | 0.880 |
| Number of reflections | 58105 | |
| <I/σ(I)> | 13.2 | 1.3 |
| Completeness [%] | 98.6 | 93.3 |
| Redundancy | 3.8 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | PROTEIN: 6 MG/ML IN 20 MM SODIUM PHOSPHATE, 75 MM NACL PH 7.4. PRECIPITANT: 0.1 M TRISHCL (PH 8.5), 2.3 M (NH4)2SO4, 10% GLYCEROL (V/V). ROOM TEMPERATURE. |
