2JA3
Cytoplasmic Domain of the Human Chloride Transporter ClC-5 in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 126.567, 149.308, 81.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.830 - 3.050 |
| R-factor | 0.28 |
| Rwork | 0.278 |
| R-free | 0.31700 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.948 |
| Data reduction software | DENZO |
| Data scaling software | TRUNCATE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.560 | 3.160 |
| High resolution limit [Å] | 2.010 | 3.050 |
| Rmerge | 0.090 | 0.420 |
| Number of reflections | 45674 | |
| <I/σ(I)> | 17.1 | 1.98 |
| Completeness [%] | 93.7 | 64.2 |
| Redundancy | 7.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 200 MM (NH4)2SO4, 10 MM TRIS HCL (PH 7.4) AND 18 % PEG 4000 |
