2J9W
Structural insight into the ESCRT-I-II link and its role in MVB trafficking
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.997, 89.713, 40.384 |
| Unit cell angles | 90.00, 95.13, 90.00 |
Refinement procedure
| Resolution | 44.860 - 1.300 |
| R-factor | 0.202 |
| Rwork | 0.200 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SE DATASET FROM SEMET SUBSTITUTED CRYSTAL |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.502 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.860 | 2.230 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.100 | 0.330 |
| Number of reflections | 8573 | |
| <I/σ(I)> | 10.57 | 3.28 |
| Completeness [%] | 88.3 | 45.7 |
| Redundancy | 4.3 | 1.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 15-25% PEG 600, 0.15M POTASSIUM THIOCYANATE, 0.2M SODIUM CHLORIDE, 0.1M GUANIDINE HYDROCHLORIDE, 0.1M SODIUM CACODYLATE PH 6.5 |
