2J9U
2 Angstrom X-ray structure of the yeast ESCRT-I Vps28 C-terminus in complex with the NZF-N domain from ESCRT-II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-14 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 66.645, 99.961, 115.619 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.030 - 2.000 |
| R-factor | 0.224 |
| Rwork | 0.223 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ZN PEAK DATASET FROM NATIVE CRYSTAL |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.703 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.470 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.100 | 0.490 |
| Number of reflections | 32191 | |
| <I/σ(I)> | 4.2 | 1.4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.02 | 6.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 20% ETHANOL, 0.1M KCL, pH 7.40 |
