2J98
Human coronavirus 229E non structural protein 9 cys69ala mutant (Nsp9)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 26.396, 61.377, 107.309 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.680 - 1.800 |
| R-factor | 0.218 |
| Rwork | 0.214 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qz8 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.639 |
| Data reduction software | HKL (SUITE) |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.800 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.080 | 0.500 |
| Number of reflections | 17889 | |
| <I/σ(I)> | 26.9 | 3.07 |
| Completeness [%] | 94.0 | 44.6 |
| Redundancy | 8 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 0.1M NA ACETATE PH 4.6 30% PEG MME 2000 |
