2J7N
Structure of the RNAi polymerase from Neurospora crassa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.021, 122.553, 114.701 |
| Unit cell angles | 90.00, 108.90, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.300 |
| R-factor | 0.219 |
| Rwork | 0.217 |
| R-free | 0.26400 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.524 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | |
| Number of reflections | 114252 | |
| <I/σ(I)> | 18.2 | 1.3 |
| Completeness [%] | 97.9 | 100 |
| Redundancy | 15.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM TRISHCL PH 7.5, 150 MM NACL, 8% PEG 6000, AND 5 MM MGCL2. |
