2J7K

Crystal structure of the T84A mutant EF-G:GDPCP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-21
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	77.230, 88.680, 114.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.000 - 2.900
Rwork	0.226
R-free	0.28800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bm0
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.070	0.470
Number of reflections	17014
<I/σ(I)>	18.6	3.2
Completeness [%]	95.4	98.7
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.3		17 % PEG8000 100 MM HEPES 46 MM TRIS 10 MM MGCL2 10 MM GDPCP, pH 7.3