2J72
alpha-glucan recognition by a family 41 carbohydrate-binding module from Thermotoga maritima pullulanase PulA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.429, 37.179, 57.213 |
| Unit cell angles | 90.00, 112.38, 90.00 |
Refinement procedure
| Resolution | 52.700 - 1.490 |
| R-factor | 0.168 |
| Rwork | 0.165 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j71 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.944 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.810 | 1.540 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.060 | 0.400 |
| Number of reflections | 33245 | |
| <I/σ(I)> | 11.9 | 2.8 |
| Completeness [%] | 97.5 | 94.5 |
| Redundancy | 3.97 | 3.37 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
