2J6Y
Structural and Functional Characterisation of partner switching regulating the environmental stress response in Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-06 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.157, 47.831, 94.968 |
| Unit cell angles | 90.00, 104.36, 90.00 |
Refinement procedure
| Resolution | 42.910 - 1.850 |
| R-factor | 0.186 |
| Rwork | 0.183 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w53 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.757 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | ARP/wARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.110 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.060 | 0.440 |
| Number of reflections | 35972 | |
| <I/σ(I)> | 5.3 | 1.7 |
| Completeness [%] | 96.1 | 93.1 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 10 % PEG 20000, 100 MM MES PH 6.5 |
