2J6V
Crystal structure of the DNA repair enzyme UV Damage Endonuclease
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97800,0.97850,0.91840 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.352, 48.700, 68.763 |
| Unit cell angles | 106.14, 94.36, 114.20 |
Refinement procedure
| Resolution | 20.000 - 1.550 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.21200 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.508 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AFRO |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.640 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.040 | 0.320 |
| Number of reflections | 56321 | |
| <I/σ(I)> | 20.2 | 3 |
| Completeness [%] | 73.8 | 23.6 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 1 X PHOSPHATE BUFFERED SALINE, pH 7.5 |
