2J6T
Ternary complex of Sulfolobus solfataricus Dpo4 DNA polymerase, O6- methylguanine modified DNA, and dATP.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-08-26 |
Detector | BRUKER |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 95.012, 102.949, 53.013 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.500 - 2.600 |
R-factor | 0.237 |
Rwork | 0.237 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bqr |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.500 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.110 | 0.970 |
Number of reflections | 16550 | |
<I/σ(I)> | 9.94 | 1.4 |
Completeness [%] | 99.6 | 98.6 |
Redundancy | 4.35 | 3.26 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.4 | 10% PEG 3350, 100 MM CA(CH3CO2)2, 50MM NACL, 5MM CACL2, AND 1MM DATP., pH 7.40 |