2J6S
Ternary complex of Sulfolobus solfataricus Dpo4 DNA polymerase, O6- methylguanine modified DNA, and dATP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-22 |
| Detector | BRUKER |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 93.682, 103.271, 52.674 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.090 - 2.500 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c22 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.100 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.670 |
| Number of reflections | 18215 | |
| <I/σ(I)> | 14.36 | 2.51 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 6.69 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 7.5% PEG 3350, 25 MM TRIS_HCL PH 7.4, 100 MM CA(CH3CO2)2, 50MM NACL, 2.5% GLYCEROL, 5MM CACL2, AND 1MM DATP. |
