2J6L
Structure of aminoadipate-semialdehyde dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-12 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.289, 162.326, 159.016 |
| Unit cell angles | 90.00, 94.17, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.300 |
| R-factor | 0.14 |
| Rwork | 0.137 |
| R-free | 0.18900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nzx |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.473 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.200 | 1.400 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.090 | 0.410 |
| Number of reflections | 816066 | |
| <I/σ(I)> | 8.91 | 1.61 |
| Completeness [%] | 85.1 | 44 |
| Redundancy | 3.33 | 0.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.6 | 0.1 M BTPROP PH 6.6, 0.20 M NABR, 23% PEG3350, 10% ETHYLENE GLYCOLE |
