2J5W
Ceruloplasmin revisited: structural and functional roles of various metal cation binding sites
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 209.140, 209.139, 82.752 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 68.520 - 2.800 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kcw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.394 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.300 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.070 | 0.320 |
| Number of reflections | 51383 | |
| <I/σ(I)> | 6.4 | 3.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | PH 4.5 |
