2J5V

GLUTAMATE 5-KINASE FROM ESCHERICHIA COLI COMPLEXED WITH GLUTAMYL-5-PHOSPHATE AND PYROGLUTAMIC ACID

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM16
Synchrotron site	ESRF
Beamline	BM16
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-10
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	101.455, 101.455, 178.728
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	88.390 - 2.500
R-factor	0.195
Rwork	0.192
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ako
RMSD bond length	0.011
RMSD bond angle	1.353
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.800	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.070	0.420
Number of reflections	61566
<I/σ(I)>	6.8	1.7
Completeness [%]	99.5	99.5
Redundancy	11.1	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		1.6 M MGSO4, 0.1 M KCL, 0.1 M MES PH 6.5