2J4T

Biological and Structural Features of Murine Angiogenin-4, an Angiogenic Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	97.472, 31.422, 95.471
Unit cell angles	90.00, 118.85, 90.00

Refinement procedure

Resolution	50.000 - 2.020
R-factor	0.229
Rwork	0.229
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bwk
RMSD bond length	0.006
RMSD bond angle	1.420
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.090
High resolution limit [Å]	2.020	2.020
Rmerge	0.140	0.520
Number of reflections	17076
<I/σ(I)>	11.3	2.8
Completeness [%]	74.2	49.1
Redundancy	2.08	0.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		INITIAL, TWINNED CRYSTALS WERE GROWN BY MIXING EQUAL VOLUMES OF PROTEIN SOLUTION (8 MG ML IN WATER) AND RESERVOIR (25 % PEG 3350, 0.2 M LI2SO4, 0.1 M TRIS-HCL, PH 8.5). THESE CRYSTALS WERE USED TO SEED PREEQUILIBRATED DROPS CONTAINING 4 MG ML PROTEIN, 20 % PEG 3350, 0.05 M LI2SO4, 0.09 M TRIS-HCL, PH 8.5.