2J3P
crystal structure of rat FGF1 at 1.4 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Collection date | 2006-05-19 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.882, 61.655, 88.856 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.640 - 1.400 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1afc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.210 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0016) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.080 | 0.210 |
| Number of reflections | 47203 | |
| <I/σ(I)> | 4.3 | 3.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.49 | 7.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 1.6 M AMMONIUM SULFATE, 0.1 M CITRIC ACID PH 5.0 |
