2J3K

Crystal structure of Arabidopsis thaliana Double Bond Reductase (AT5G16970)-Ternary Complex II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	277
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	49.080, 122.450, 147.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.800
R-factor	0.199
Rwork	0.199
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.025
RMSD bond angle	4.600
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800
Rmerge	0.050
Number of reflections	23815
Completeness [%]	82.6
Redundancy	5.38

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			reservoir solution containing 20% (w/v) polyethylene glycol 3350 and 0.2 M potassium chloride