2J2C
Crystal structure of Human Cytosolic 5'-Nucleotidase II (NT5C2, cN-II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-22 |
Detector | ADSC CCD |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 91.465, 128.034, 130.415 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.200 |
R-factor | 0.154 |
Rwork | 0.152 |
R-free | 0.18400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bde |
RMSD bond length | 0.016 |
RMSD bond angle | 1.428 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 91.290 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.060 | 0.150 |
Number of reflections | 39212 | |
<I/σ(I)> | 22.5 | 11.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 1.8 M MG2SO4 0.1 M TRIS PH 8.5 |