2J21

Human p53 core domain mutant M133L-V203A-N239Y-N268D-R282W

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX10.1
Synchrotron site	SRS
Beamline	PX10.1
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	64.813, 71.398, 104.978
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.200 - 1.600
R-factor	0.198
Rwork	0.198
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uol
RMSD bond length	0.009
RMSD bond angle	1.500
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.200	1.700
High resolution limit [Å]	1.600	1.600
Rmerge	0.060	0.360
Number of reflections	64960
<I/σ(I)>	18.3	4.3
Completeness [%]	99.9	100
Redundancy	6.3	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1