2J0V
The crystal structure of Arabidopsis thaliana RAC7-ROP9: the first RAS superfamily GTPase from the plant kingdom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 76.760, 30.200, 139.360 |
| Unit cell angles | 90.00, 100.06, 90.00 |
Refinement procedure
| Resolution | 37.800 - 1.780 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mh1 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.633 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.180 | 1.880 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.080 | 0.440 |
| Number of reflections | 61628 | |
| <I/σ(I)> | 12.3 | 3.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 50 MM BISTRIS PH 6.5, 10 MM MGCL2, 5 MM DTT, 200 MM KCL, 10% (W/V) PEG 2000, 10% (W/V) PEG 3350 |
