2J06
Crystal structure of the RasGAP SH3 domain at 1.8 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-19 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 |
| Unit cell lengths | 33.610, 33.610, 196.330 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.110 - 1.800 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.24000 |
| Structure solution method | SAD |
| RMSD bond length | 0.027 |
| RMSD bond angle | 3.400 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.360 |
| Number of reflections | 23136 | |
| <I/σ(I)> | 21.8 | 4.9 |
| Completeness [%] | 96.2 | 81.8 |
| Redundancy | 4.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 90 MM KNO3, 15.5% GLYCEROL, 90 MM TAPS, PH 9 AND 45.5% PEG8000 |
