2IZT
Structure of casein kinase gamma 3 in complex with inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-04-10 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 56.433, 56.433, 220.266 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.350 - 2.000 |
| R-factor | 0.225 |
| Rwork | 0.222 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2chl |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.527 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.700 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.360 |
| Number of reflections | 26655 | |
| <I/σ(I)> | 10.9 | 2.4 |
| Completeness [%] | 93.0 | 87.6 |
| Redundancy | 4.3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.05 M CACL2, 20% PEG6000, 10% ETHYLENE GLYCOL, 0.10 M TRIS PH=8.0, pH 8.00 |
