2IYA
The crystal structure of macrolide glycosyltransferases: A blueprint for antibiotic engineering
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2005-07-13 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.338, 103.471, 74.590 |
| Unit cell angles | 90.00, 94.82, 90.00 |
Refinement procedure
| Resolution | 74.540 - 1.700 |
| R-factor | 0.168 |
| Rwork | 0.167 |
| R-free | 0.19800 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.340 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.710 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.080 | 0.260 |
| Number of reflections | 81807 | |
| <I/σ(I)> | 13.7 | 4 |
| Completeness [%] | 96.1 | 80.1 |
| Redundancy | 4.2 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25-26% PEG2KMME, 0.8M NAFORMATE, 0.1M NAACETATE PH 4.6 |
