2IY2
Crystal structure of the N-terminal dimer domain of E.coli DsbG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2005-10-23 |
Detector | MARRESEARCH |
Spacegroup name | P 41 |
Unit cell lengths | 56.842, 56.842, 42.049 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.790 - 1.900 |
R-factor | 0.198 |
Rwork | 0.196 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1v58 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.441 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CCP4 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.120 | 0.310 |
Number of reflections | 10806 | |
<I/σ(I)> | 9.3 | 3.3 |
Completeness [%] | 99.4 | 99.6 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.2M CADMIUM CHLORIDE, 33% MPD, pH 7.50 |