2IXU

Crystal structure of the modular Cpl-1 endolysin complexed with a peptidoglycan analogue (wild-type endolysin)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-07-14
Detector	MAR scanner 345 mm plate
Spacegroup name	C 2 2 21
Unit cell lengths	80.521, 96.504, 127.317
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.320 - 2.280
R-factor	0.21236
Rwork	0.212
R-free	0.26238
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h09
RMSD bond length	0.021
RMSD bond angle	1.566
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.860	2.410
High resolution limit [Å]	2.280	2.260
Rmerge	0.070	0.430
Number of reflections	32678
<I/σ(I)>	6.6	1.8
Completeness [%]	99.9	99.5
Redundancy	9.3	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		pH 6.0