2IX7
Structure of apo-calmodulin bound to unconventional myosin V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 112.000, 112.000, 102.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.800 - 2.500 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.25900 |
| Structure solution method | MIRAS |
| Starting model (for MR) | 1wcd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.800 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.070 | 0.250 |
| Number of reflections | 25837 | |
| <I/σ(I)> | 8.4 | 2.8 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5.9 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 277 | VAPOR DIFFUSION METHOD (4 C) PROTEIN SOLUTION; 10MG/ML IN 10MM IMIDAZOL PH7.0, 20MM NACL. RESERVOIR; 1.8M SA, 50MM MES PH5.0, 5% MPD, 5MM EGTA, 2MM NAN3, pH 5.00 |
