2IWZ
Human mitochondrial beta-ketoacyl ACP synthase complexed with hexanoic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-31 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.912, 95.920, 114.637 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.310 - 1.650 |
| R-factor | 0.156 |
| Rwork | 0.155 |
| R-free | 0.18800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w0i |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.277 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.300 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.480 |
| Rmerge | 0.090 | 0.740 |
| Number of reflections | 132820 | |
| <I/σ(I)> | 5.4 | 1 |
| Completeness [%] | 97.0 | 79.7 |
| Redundancy | 5.4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 24% PEG3350, 0.2M NH4CL, pH 7.80 |
