2IWV
Structure of the monomeric outer membrane porin OmpG in the open and closed conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-12 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.600, 77.000, 103.900 |
| Unit cell angles | 79.30, 73.40, 74.30 |
Refinement procedure
| Resolution | 14.980 - 2.300 |
| R-factor | 0.226 |
| Rwork | 0.224 |
| R-free | 0.26900 |
| Structure solution method | OTHER |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.504 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.100 |
| Number of reflections | 240777 |
| <I/σ(I)> | 7.56 |
| Completeness [%] | 90.6 |
| Redundancy | 2.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.9 | 100 MM HEPES PH 7.5 30% PEG 4000 200 MM CACL2 |
