2IWL
Structure of the PX Domain of Phosphoinositide 3-Kinase-C2alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 4 3 2 |
| Unit cell lengths | 115.933, 115.933, 115.933 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.000 - 2.600 |
| R-factor | 0.252 |
| Rwork | 0.247 |
| R-free | 0.29000 |
| Structure solution method | SIR |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.654 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.090 | 0.480 |
| Number of reflections | 357186 | |
| <I/σ(I)> | 45.1 | 1.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 41 | 42.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 1.7 M LI2SO4, 0.05 M MES PH 5.6 |
