2IVM
Crystal structure of a transcriptional regulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 100.827, 100.827, 99.503 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.070 - 2.500 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.25500 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.584 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.650 |
| Number of reflections | 18352 | |
| <I/σ(I)> | 19.2 | 4.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.1 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.5 |
